31st Nano Congress for Future Advancements
Pharmaceutical Technology Department, National Research Centre, Egypt
Title: Bilosomes as a novel ocular drug delivery system for acetazolamide: In vitro and in vivo evaluation
Biography: Amira Mohsen
Acetazolamide (ACZ), a carbonic anhydrase inhibitor, is used to reduce the eye pressure in people suffering from glaucoma. ACZ has limited aqueous solubility and poor corneal permeation. The objective of the present study was to attain enhanced ocular delivery of ACZ via its incorporation into bilosomes. ACZ loaded bilosomes were prepared by the thin film hydration technique employing Span 60, cholesterol and different bile salts (sodium cholate, sodium deoxycholate, sodium taurocholate and sodium tauroglycocholate). They were further characterized via particle size and zeta potential analysis in addition to transmission electron microscopy. In vitro release studies were performed using diffusion bag technique. The developed formulations exhibited high entrapment efficiencies up to 74.23. They were spherical in shape and their sizes were in the nanometric dimensions ranging from 349.8 nm to 734.6 nm with negatively charged zeta potential values (<-43.4 mV). In vitro drug release profiles revealed sustained release of the drug up to 8 hours. In vivo pharmacodynamic assessment of the optimized ACZ bilosomal formulation, employing male albino rabbits, revealed enhanced and prolonged intraocular pressure lowering effect compared to plain ACZ suspension, marketed ACZ oral tablets as well as marketed dorzolamide eye drops. Furthermore, in vivo ocular irritancy test proved the safety of the optimized bilosomal formulation after ocular application.