Xiaoling Lu
University of Applied Sciences Kaiserslautern, Germany
Title: Reduced graphene-oxide based in-line impedimetric biosensor for detecting prostate cancer specific antigen
Biography
Biography: Xiaoling Lu
Abstract
Chemically exfoliated graphene-oxide (GO) is being exploited due to its similarity to graphene and tested out as an alternative to overcome the dilemma that graphene is facing towards wafer-scale and robust device preparation. In this work, we prepared GO thin-films in the manner of wafer scale by the spin-coat technique on the top of interdigitated electrodes (IDEs) with glass as substrates. The isolated GO thin-films are transformed into conductive rGO thin-films by thermal reduction. The residual -COOH groups on the surface of rGO thin film provide diverse possibilities of chemical functionalization to covalently immobilize the receptor molecules. An in-line impedimetric spectroscopy based rGO thin-films as transducer layers are tested out for label-free detection of Prostate Cancer Specific Antigen (PSA). This established biosensor exhibits ultra-sensitivity and announced sensing range because of the combination effect of tunable fermi level and fast charge/discharge behaviour of nanocapcitors. The pronounced PSA detection scale ranges from 33 fM to 330 nM at frequency 1000 Hz.